Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Muenzaniso Nhamba: VD105E
Abbr.:IBRV Ab Elisa
Muenzaniso: serum

Tsanangudzo

Tsanangudzo

BVDV Ab Elisa

Chinangwa Kushandiswa: InfectiousBovine Rhinotracheitis VirusAntibody ELISA Detection Kit inoshandiswakuziva chinotapukira rhinotracheitishutachiona hwehutachiona mu bovine serum in vitro.

Muenzaniso: serum

Zvishandiso Zvakapihwa:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Kuedza Maitiro & Dudziro

STEP 1 Test Procedure

1.1 Wash Solution Kugadzirira

Working concentration washing liquid:Usati washandiswa, mushonga wekugeza unofanirwa kudzorerwa kukushisa kwekamuri (20-25 degree) uye inozununguswa zvakafanana kuti iite yunifomu. Wobva wawedzera ka20 iyo yakaomeswa yekugezesa mhinduro nemvura yakacheneswa (somuenzaniso, 5ml yakaomeswa yekugeza mhinduro inowedzerwa kune 95ml yakanatswa mvura), sanganisa zvakanaka, uye yakanyungudutswa yekuwachisa mvura inogona kuchengetwa pa2-8 dhigirii kwemazuva manomwe.

1.2 Muenzaniso Kugadzirira

① Ropa rose remhuka rakatorwa, rakaiswa centrifuged pa4000rpm kwemaminetsi gumi, uye supernatant yakaunganidzwa. Serum inogonawo kubatanidzwa zvakasununguka pasina hemolysis.

② Diraisa serum kuti iedzwe ka50 muserum dilution plate (semuenzaniso: wedzera 3ul yeserum kuti iongororwe kune 147ul sampuli diluent).

Cherechedza: Zvidzore zvisina kunaka uye zvakanaka hazvifanirwe kuderedzwa

1.3 Test Procedure

①Tora iyo enzyme label Microplate (maererano nenhamba yemasampuli, inogona kupatsanurwa uye kushandiswa kakawanda), wedzera 100ul ye diluted serum kune iyo enzyme label Microplate, isa matsime maviri ega ega ekudzora kwakashata uye kutonga kwakanaka, uye wedzera 100ul kune mhinduro zvakanaka zvichiteerana. Zvinyoro zunza muenzaniso mugomba, uchishandisa firimu rekuvhara ndiro kuvhara ndiro, uye incubate pa 37℃kwemaminitsi makumi matatu.

②Vhura firimu rekuvharisa ndiro, zunza mhinduro mundiro zvakanaka, wedzera 300µl yemvura yekugeza yekushanda patsime rega rega, uye wobva warasa mvura mutsime. Dzokorora matanho ari pamusoro apa ka5, uye pakupedzisira pat yakaoma papepa rakachena rinopinza.

③Diluted enzyme conjugate: Dilute the enzyme conjugate (80 ×) 80 nguva (somuenzaniso: 10ul enzyme conjugate concentrate + 790 ul sample diluent, iyo chaiyo mari inodiwa inoverengwa maererano nenhamba yemasampuli ekuedza), sanganisa zvakanaka uye uise parutivi.

④Wedzera 100µl yeEnzyme Conjugate kune rimwe nerimwe tsime, vhara ndiro, uye incubate pa 37℃kwemaminitsi makumi matatu.

⑤Washing enzyme plate, iyo chaiyo mashandiro akafanana neari pamusoro ②.

⑥Wedzera 100µl substrate mhinduro kutsime rega rega, vhara ndiro uye incuite kwemaminetsi gumi pa37℃kure nechiedza.

STEP 2 Result Dudziro

Iyo OD450 kukosha kwetsime rega rega yakaverengwa pa96-tsime microplate muverengi.

2.1 Validation Avhareji yeOD450nm kukosha kwekutonga kwakashata yaive <0.25 uye yepakati OD450nm kukosha kwekutonga kwakanaka yaive Yakakura kupfuura kana kuenzana ne0.6, mhedzisiro yekuedza yaive yechokwadi. Kana zvisina kudaro, muedzo unofanirwa kudzokororwa.

2.2 Maitiro ekuverenga

Pamuenzaniso wega wega, verenga iyo S/P nenzira inotevera uchishandisa yakagadziridzwa sampuli uye kutonga maitiro:

product-554-49

2.3 Dudziro yemhedzisiro

Kana S/P <0.2, inotongwa seisina kunaka.

Kana S/P> 0.3, inotongwa seyakanaka.

Kana 0.2 Zvishoma pane kana kuenzana neS/P Zvishoma pane kana kuenzana na 0.3, zvinoonekwa sezviri kufungira, uye zvinokurudzira kudzokorora sampling uye kuyedza vhiki imwe gare gare.

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