Inotapukira Bursal Chirwere Hutachiona Antibody ELISA Detection Kit
Muenzaniso Nhamba: VD501E
Abbr.:IBDV Ab Elisa
Muenzaniso: serum
Tsanangudzo
Tsanangudzo
IBDV Ab Elisa
Chinangwa Kushandiswa:
Infectious Bursal Disease Virus Antibody ELISA Detection kit inoshandiswa kuona Infectious Bursal Disease Virus antibodies mu chicken serum in vitro.
Muenzaniso:serum
Zvishandiso Zvakapihwa:
Kuedza Maitiro & Dudziro
STEP 1 Test Procedure
1.1 Wash Solution Kugadzirira
Working concentration washing liquid: Usati washandiswa, sarudzo yekushambidza inofanira kudzorerwa kumhepo yekushisa (20-25 degree) uye inozununguswa zvakafanana kuti iite yunifomu. Wobva wawedzera ka20 iyo yakaomeswa yekugezesa mhinduro nemvura yakacheneswa (somuenzaniso, 5ml yakaomeswa yekugeza mhinduro inowedzerwa kune 95ml yakanatswa mvura), sanganisa zvakanaka, uye yakanyungudutswa yekuwachisa mvura inogona kuchengetwa pa2-8 dhigirii kwemazuva manomwe.
1.2 Muenzaniso Kugadzirira
① Ropa rose remhuka rakatorwa, rakaiswa centrifuged pa4000rpm kwemaminetsi gumi, uye supernatant yakaunganidzwa. Serum inogonawo kubatanidzwa zvakasununguka pasina hemolysis.
②Dilute serum kuti iongororwe ka25mu serum dilution plate (semuenzaniso:wedzera 6ul ye serum kuti iongororwe ku144ulsampuli diluent).
Cherechedza: Zvidzore zvisina kunaka uye zvakanaka hazvifanirwe kuderedzwa.
1.3 Test Procedure
①Tora iyo enzyme label Microplate (maererano nenhamba yemasampuli, inogona kupatsanurwa uye kushandiswa kakawanda), wedzera 100ul ye diluted serum kune iyo enzyme label Microplate, isa matsime maviri ega ega ekudzora kwakashata uye kutonga kwakanaka, uye wedzera 100ul kune mhinduro zvakanaka zvichiteerana. Zvinyoro zunza muenzaniso mugomba, uchishandisa firimu rekuvhara ndiro kuvhara ndiro, uye incubate pa 37℃kwemaminitsi makumi matatu.
②Vhura firimu rekuvharisa ndiro, zunza mhinduro mundiro zvakanaka, wedzera 300µl yemvura yekugeza yekushanda patsime rega rega, uye wobva warasa mvura mutsime. Dzokorora matanho ari pamusoro apa ka5, uye pakupedzisira pat yakaoma papepa rakachena rinopinza.
③Wedzera 100µl yeEnzyme Conjugate kutsime rega rega, vhara ndiro, uye incubate pa37℃kwemaminitsi makumi matatu.
④Vhura firimu repureti rekuvhara, zunza mhinduro mundiro zvakanaka, wedzera 300µl yemvura yekugeza yekushanda patsime rega rega, uye wobva warasa mvura mutsime. Dzokorora matanho ari pamusoro apa ka5, uye pakupedzisira pat yakaoma papepa rakachena rinopinza.
⑤Wedzera 100µl substrate mhinduro kutsime rega rega, vhara ndiro uye incubate kwemaminetsi gumi pa 37℃kure nechiedza.
⑥Wedzera 50µl yekumisa mhinduro kutsime rega rega, uye tarisa mhedzisiro mukati memaminetsi mashanu.
STEP 2 Result Dudziro
Iyo OD450 kukosha kwetsime rega rega yakaverengwa pa96-tsime microplate muverengi.
2.1 Kusimbiswa
Avhareji yeOD450nm kukosha kwekutonga kwakashata yaive <0.15, uye avhareji OD450nm kukosha kwekutonga kwakanaka / avhareji OD450nm kukosha kwekutonga kwakashata yaive Yakakura kupfuura kana yakaenzana ne3.5, mhedzisiro yekuyedza yaive yechokwadi. Kana zvisina kudaro, muedzo unofanirwa kudzokororwa.
2.2 Maitiro ekuverenga
Pamuenzaniso wega wega, verenga iyo S/P nenzira inotevera uchishandisa yakagadziridzwa sampuli uye kutonga maitiro:
S / P{0}(ODSAMPLE-ODNC) / (ODPC-ODNC)
2.3 Dudziro yemhedzisiro
Kana S/P <0.3, inotongwa seisina kunaka.
Kana S/P> 0.35, inotongwa seyakanaka.
Kana 0.3 Zvishoma pane kana kuenzana neS/P Zvishoma pane kana kuenzana na 0.35, zvinoonekwa sezviri kufungira, uye zvinokurudzira kudzokorora sampling uye kuyedza vhiki imwe gare gare.
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